Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

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PUBLICATION TYPE:

Journal Article

AUTHORS:

Jain, Piyush K.; Ramanan, Vyas; Schepers, Arnout G.; Dalvie, Nisha S.; Panda, Apekshya; Fleming, Heather E.; Bhatia, Sangeeta N.

SOURCE:

Angewandte Chemie International Edition (2016)

Access:

Manuscript (PDF)

Journal: https://pubmed.ncbi.nlm.nih.gov/27554600/

ABSTRACT:

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

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